Journal: Cell reports

Article Title: Recursive feedback between matrix dissipation and chemo-mechanical signaling drives oscillatory growth of cancer cell invadopodia

doi: 10.1016/j.celrep.2021.109047

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 280 kDa Matrigel Corning Cat# 354230 Calcium Sulfate (CaSO4) Sigma Aldrich Cat# 255696-10G Lipofectamine 3000 ThermoFisher Scientific Cat# L3000075 OptiMEM ThermoFisher Scientific Cat# 31985062 EGF Peprotech, Inc. Cat# AF-100-15 Puromycin ThermoFisher Scientific Cat# A1113803 Neomycin ThermoFisher Scientific Cat# 21810031 Fluoresceinamine Sigma Aldrich Cat# 20162 Critical commercial assays MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 GeneJet Plasmid Miniprep kit ThermoFisher Scientific Cat# K0701 Deposited data Measured invadopodia dynamics of MDA-MB-231 This paper https://doi.org/10.17632/tp7fkkxk44.2 Measured circularity of HT-1080 cells Wisdom et al., 2018 https://doi.org/10.17632/tp7fkkxk44.2 Experimental models: Cell lines MDA-MB-231 ATCC ATCC® HTB-26 HT-1080 cells ATCC ATCC® CCL-121 Recombinant DNA RFP-LifeAct Gift from A. Dunn N/A Software and algorithms Simulation of invadopodia dynamics This paper N/A High throughput invadopodia dynamics analysis Chang et al., 2020 N/A MATLAB Mathworks https://www.mathworks.com/products/matlab.html Imaris Oxford Instruments https://imaris.oxinst.com/ ImageJ ImageJ https://imagej.net/Welcome Other Stress-controlled rheometer TA Instruments AR2000EX Open in a separate window KEY RESOURCES TABLE A chemo-mechanical model for invadopodia dynamics in 3D matrices is developed Oscillations occur when timescales for extension and myosin dynamics are comparable Matrix plastic strain accumulates by cyclic ratcheting during invadopodia growth High matrix plasticity facilitates the oscillatory growth of invadopodia

Techniques: Blocking Assay, Virus, Recombinant, Modification, Plasmid Preparation, Software, High Throughput Screening Assay

Journal: Cell reports

Article Title: D1 and D2 neurons in the nucleus accumbens enable positive and negative control over sugar intake in mice

doi: 10.1016/j.celrep.2023.112190

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit-anti-mCherry Abcam RRID: AB_2571870 TRITC-conjugated affinipure Goat anti-Rabbit IgG (H + L) Jackson ImmunoResearch Labs RRID: AB_2337932 Goat Anti-GFP antibody (FITC) Abcam RRID: AB_305635 Anti-c-Fos Rabbit polyclonal to c-Fos Abcam RRID: AB_2231989 Biotinylated Goat Anti-Rabbit IgG antibody Vector Laboratories RRID: AB_2313606 Chemicals, peptides, and recombinant proteins Sucralose Sigma PubChem Substance ID: 329769983 Rebaudioside A Sigma PubChem Substance ID: 329747271 Glucose Sigma PubChem Substance ID: 24895335 Methyl α-D-glucopyranoside Sigma PubChem Substance ID: 24897328 Denatonium Benzoate Sigma PubChem Substance ID: 57654112 Lithium chloride Sigma PubChem Substance ID: 24896459 Victoza (liraglutide) injection Novo Nordisk N/A DiI lipophiliccarbocyanine dye Sigma PubChem Substance ID: 57650110 PubChem Substance ID: Invitrogen Cat #D1817 Dextran, Tetramethylrhodamine, 10,000 MW, Lysine Fixable (Fluoro-Ruby) Critical commercial assays VECTASTAIN Elite ABC HRP Kit (Peroxidase, Standard) Vector Laboratories Cat# PK-6100 C&B Metabond Parkell SKU: S380 Experimental models: Organisms/strains Mouse: B6.FVB(Cg)-Tg(Drd1-cre)EY262Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_030989-UCD Mouse: B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_032108-UCD Mouse: B6(Cg)-Tg(Phox2b-cre)3Jke/J The Jackson Laboratory RRID:IMSR_JAX:016223 Mouse: B6; 129S6-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007905 Recombinant DNA and virus strains H129 CAG-LSL-TK-tdTomato NIH Center for Neuroanatomy with Neurotropic Virus ΔTK-TT PRV CMV-EGFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-152 PRV CAG-DIO-TK-GFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-2017 AAV-flex-taCasp3-TEVp Addgene Cat # 45580-AAV5; RRID:Addgene_45580 AAV-EF1a-DIO-hChR2(H134R)-EYFP Addgene Cat # 20298-AAV5; RRID:Addgene_20298 Software and algorithms MATLAB R2017b MathWorks https://www.mathworks.com/products/matlab/ GraphPad Prism 8 GraphPad https://www.graphpad.com/scientific-software/prism/ Adobe Illustrator CC 2017 Adobe https://shop.adobe.com/ Open Ephys GUI Open Ephys https://open-ephys.org/software Neuroscope, NDManager and Klusters Lynn Hazan & Michaël Zugaro http://neurosuite.sourceforge.net/ KlustaKwik Ken Harris http://klustakwik.sourceforge.net/ BukaLab Asapti Labs https://asapti.com/ BioRender BioRender https://biorender.com Other Implantable Optical Fibers Doric Lenses, Canada MFC_200/240–0.22_6mm_ZF1.25(G)_FLT 32 CH Microelectrode (Electrophysiological recordings) NeuroNexus A2x16–10mm-50–500-177-A32 Micro-Renathane (tubing) Braintree SKU: MRE033 Open in a separate window KEY RESOURCES TABLE.

Techniques: Plasmid Preparation, Recombinant, Injection, Software